复发癌患者外周血单个核细胞产生肝细胞生长因子的情况

Hepatocyte growth factor production by peripheral blood mononuclear cells of recurrent cancer patients.

作者信息

Beppu K, Uchiyama A, Morisaki T, Matsumoto K, Nakamura T, Tanaka M, Katano M

机构信息

Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

出版信息

Anticancer Res. 2001 May-Jun;21(3C):2195-200.

PMID:11501846

Abstract

The elevation of circulating hepatocyte growth factor (HGF) concentrations is associated with tumor progression and prognosis in cancer patients. We aimed to clarify whether peripheral blood mononuclear cells (PBMC) of cancer patients can produce circulating HGF. In sera and PBMC supematants of 39 cancer patients and 55 control subjects, we measured HGF concentrations by enzyme-linked immunosorbent assay and analyzed HGF expression in PBMC by reverse transcriptase-polymerase chain reaction and Western blotting. Compared with primary cancer patients and control subjects, recurrent cancer patients showed higher serum HGF concentrations (p < 0.05, p < 0.01 respectively), higher HGF concentrations in PBMC supernatants (p < 0.01 for both) and more frequent HGF mRNA expression in PBMC. Additionally, HGF mRNA was induced in PBMC of a control subject cultured with the sera of recurrent cancer patients. These results indicate that PBMC of recurrent cancer patients can produce HGF and that their sera may contain substances that induce HGF mRNA expression.

摘要

循环中肝细胞生长因子（HGF）浓度的升高与癌症患者的肿瘤进展及预后相关。我们旨在阐明癌症患者的外周血单个核细胞（PBMC）是否能产生循环HGF。在39例癌症患者和55例对照者的血清及PBMC上清液中，我们采用酶联免疫吸附测定法测量HGF浓度，并通过逆转录-聚合酶链反应及蛋白质免疫印迹法分析PBMC中HGF的表达。与原发性癌症患者及对照者相比，复发癌症患者的血清HGF浓度更高（分别为p < 0.05，p < 0.01），PBMC上清液中的HGF浓度更高（两者均为p < 0.01），且PBMC中HGF mRNA表达更频繁。此外，用复发癌症患者的血清培养的对照者PBMC中可诱导HGF mRNA表达。这些结果表明，复发癌症患者的PBMC能够产生HGF，且其血清中可能含有诱导HGF mRNA表达的物质。