Gamón M, Lleó C, Ten A, Mocholí F
Laboratorio Agroalimentario, Burjassot, Spain.
J AOAC Int. 2001 Jul-Aug;84(4):1209-16.
Pesticide residues in fruit and vegetables were determined by gas chromatography/tandem mass spectrometry (GC/MS/MS). Electron impact (EI)/MS/MS and chemical ionization (CI)/MS/MS were developed for 80 compounds, including organochlorine, organophosphorus, organonitrogen, and pyrethroids, providing unambiguous spectral confirmation for these complex matrixes. Residues were extracted from samples with acetone followed by a mixture of dichloromethane-petroleum ether. Two injections per sample were required for analysis of the entire pesticide list by EI/MS/MS and CI/MS/MS. Initial steps involving cleanup and concentration of extracts were eliminated. The excellent selectivity and good linearity allowed quantification and identification of low levels of pesticides in the most difficult matrixes. The method has been used for routine analysis of many vegetables.
采用气相色谱/串联质谱法（GC/MS/MS）测定水果和蔬菜中的农药残留。针对80种化合物开发了电子轰击（EI）/MS/MS和化学电离（CI）/MS/MS方法，这些化合物包括有机氯、有机磷、有机氮和拟除虫菊酯类，可为这些复杂基质提供明确的光谱确认。用丙酮从样品中提取残留，随后用二氯甲烷 - 石油醚混合物进行提取。通过EI/MS/MS和CI/MS/MS分析整个农药清单，每个样品需要进样两次。省去了提取物净化和浓缩的初始步骤。出色的选择性和良好的线性使得能够在最难分析的基质中对低含量农药进行定量和鉴定。该方法已用于多种蔬菜的常规分析。
