Johnson E W, Hughes T K, Smith E M
Department of Microbiology, University of Texas Medical Branch, Galveston, USA.
J Biol Regul Homeost Agents. 2001 Apr-Jun;15(2):156-62.
Murine mononuclear leukocytes express adrenocorticotropin (ACTH) receptors that were recognized by a monospecific antiserum to the ACTH receptor on Y-1 adrenal cells. The antiserum was utilized in an immunofluorescence (IF) assay to characterize the distribution of ACTH receptors on resting murine mononuclear leukocyte populations. Forty-seven percent of spleen cells, 32% of lymph node cells, and 1% of thymocytes constitutively expressed ACTH receptors. Separation of lymphocytes into purified B cell and T cell populations, followed by IF analysis revealed that 47% of B cells and 23% of T cells possessed ACTH receptors. Helper T cells (CD4+ T cells) constituted the majority of ACTH receptor-positive T lymphocytes. Furthermore, 47% of resident peritoneal macrophages, purified by adherence to plastic, expressed ACTH receptors. The T-lymphocyte mitogen, concanavalin A, interferon gamma, and ACTH enhanced ACTH receptor expression. The differential distribution of ACTH receptor-positive cells among specific leukocyte populations explains in part why differential cellular responses are observed and implies important regulatory functions for these receptors in the generation or regulation of immune responses.
小鼠单核白细胞表达促肾上腺皮质激素（ACTH）受体，该受体可被针对Y-1肾上腺细胞上ACTH受体的单特异性抗血清识别。该抗血清用于免疫荧光（IF）测定，以表征静息小鼠单核白细胞群体上ACTH受体的分布。47%的脾细胞、32%的淋巴结细胞和1%的胸腺细胞组成性表达ACTH受体。将淋巴细胞分离为纯化的B细胞和T细胞群体，然后进行IF分析，结果显示47%的B细胞和23%的T细胞具有ACTH受体。辅助性T细胞（CD4+T细胞）构成了ACTH受体阳性T淋巴细胞的大多数。此外，通过贴壁于塑料纯化的47%的驻留腹膜巨噬细胞表达ACTH受体。T淋巴细胞丝裂原、伴刀豆球蛋白A、干扰素γ和ACTH可增强ACTH受体表达。ACTH受体阳性细胞在特定白细胞群体中的差异分布部分解释了为何会观察到不同的细胞反应，并暗示这些受体在免疫反应的产生或调节中具有重要的调节功能。
