Borgers M, De Nollin S, Thoné F
Histochemistry. 1975 Jun 5;43(3):257-67. doi: 10.1007/BF00499707.
The development of alkaline phosphatase during invasion and encystment of Trichinella spiralis in rat skeletal muscle fibres was studied at the ultrastructural level. On day 14 after infection, the enzymatic activity is found in proliferating parts of the T-tubular system and in parts of the plasmalemma. In cells, in which a strong hyperplasia of this system is noted. AlPase is present in the abundant network of stratified and concentric membranes from which a large number of pinocytic vesicles arise. From day 50 till 1 year after infection the enzyme activity was invariably present in the matrix surrounding the larvae and was confined to the enormous amounts of cytoplasmic membranes. The possible functional significance of this enzyme in the matrix, in view of its peculiar localization in the immediate vicinity of the parasite, is discussed. In the presence of 0.1 mM of the levamisole analogue, compound R 30402, which is a stereospecific inhibitor of AlPase, the activity is completely lost.
在超微结构水平上研究了旋毛虫在大鼠骨骼肌纤维中侵袭和包囊形成过程中碱性磷酸酶的发育情况。感染后第14天，在T小管系统的增殖部位和部分质膜中发现酶活性。在该系统有强烈增生的细胞中，碱性磷酸酶存在于分层和同心膜的丰富网络中，从中产生大量的胞饮小泡。感染后第50天至1年，酶活性始终存在于幼虫周围的基质中，并局限于大量的细胞质膜。鉴于该酶在寄生虫附近的特殊定位，讨论了其在基质中的可能功能意义。在存在0.1 mM左旋咪唑类似物化合物R 30402（一种碱性磷酸酶的立体特异性抑制剂）的情况下，活性完全丧失。
