Grigoriev P A, Schlegel R, Gräfe U
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia.
Bioelectrochemistry. 2001 Aug;54(1):11-5. doi: 10.1016/s0302-4598(01)00102-7.
The macrodiolide antibiotic elaiophylin (1) forms stable, long-lasting cation selective ion channels in planar lipid bilayer membranes prepared from soybean phosphatidylcholine. Current of the single ion channel displayed two sublevels corresponding to the two substates of the channel conductance: a slow substate, with about 5 s of mean dwell time in the open state at 40% level of the total amplitude conductance, and a fast substate of higher conductance with dwell times in the open and closed state of about 0.1 s. Amplitude conductances of the single ion channels in 200 mM of LiCl, NaCl, KCl, RbCl and CsCl were 75, 140, 220, 240 and 226 pS, and the conductance was linear function of the electrolyte concentration. Ratios of cation to anion permeabilities of the channel for NaCl and KCl were 8+/-2 and >24, respectively. A molecular model of the channel structure is suggested.
大环二萜类抗生素伊来霉素（1）在由大豆磷脂酰胆碱制备的平面脂质双层膜中形成稳定、持久的阳离子选择性离子通道。单离子通道电流显示出两个亚水平，对应于通道电导的两个亚状态：一个慢速亚状态，在总幅度电导的40%水平下，开放状态的平均停留时间约为5秒；以及一个电导较高的快速亚状态，开放和关闭状态的停留时间约为0.1秒。在200 mM的LiCl、NaCl、KCl、RbCl和CsCl中，单离子通道的幅度电导分别为75、140、220、240和226 pS，且电导是电解质浓度的线性函数。该通道对NaCl和KCl的阳离子与阴离子渗透率之比分别为8±2和>24。提出了通道结构的分子模型。
