采用薄层色谱法对大鼠粪便中的色氨酸代谢产物进行定量分析。
Quantitation of tryptophan metabolites in rat feces by thin-layer chromatography.
作者信息
Anderson G M
出版信息
J Chromatogr. 1975 Feb 26;105(2):323-8. doi: 10.1016/s0021-9673(01)82261-5.
PMID:1150778
Abstract
Indole, skatole, indole-3-acetic acid, indole-3-propionic acid, tryptamine, and free tryptophan have been extracted from rat fecal samples and quantitated by thin-layer chromatography. Additional tryptophan metabolites have been recovered in yields of 87-97% from supplemented fecal samples. Detection limits for all the compounds studied were in the 0.1 mug/g feces range. Quantitation was possible in the mug/g range with an estimated accuracy of plus or minus 10%.
摘要
已从大鼠粪便样本中提取出吲哚、粪臭素、吲哚 - 3 - 乙酸、吲哚 - 3 - 丙酸、色胺和游离色氨酸，并通过薄层色谱法进行定量。从添加后的粪便样本中回收了其他色氨酸代谢产物，回收率为87 - 97%。所研究的所有化合物的检测限在0.1微克/克粪便范围内。在微克/克范围内可以进行定量，估计准确度为正负10%。
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