Oterino J, Sanchez Toranzo G, Zelarayán L, Valz-Gianinet J N, Bühler M I
Departamento de Biologia del Desarrollo, San Miguel de Tucumán, Argentina.
Zygote. 2001 Aug;9(3):251-9. doi: 10.1017/s0967199401001277.
Denuded Bufo arenarum oocytes matured in vitro by progesterone treatment exhibited abnormal segmentation due to the penetration of more than one sperm. These oocytes were able to respond to activation stimuli and exhibited the external signs characteristic of activation. However, the prevention of polyspermy was not effective in these oocytes, which exhibited numerous sperm in their cytoplasm. The aim of this work was to analyse the cortical reaction in polyspermic Bufo arenarum oocytes matured in vitro. The result indicate that the cortical reaction of these oocytes seems to occur with a chronological sequence similar to that described for ovoposited oocytes of this species. In addition, when, 1 min after pricking, cortical granule exocytosis occurred, the oocytes became refractory to sperm entry, suggesting that they are able to establish a slow block to polyspermy.
经孕酮处理在体外成熟的去除卵膜的阿根廷蟾蜍卵母细胞，由于多个精子的穿透而出现异常分裂。这些卵母细胞能够对激活刺激作出反应，并表现出激活的外部特征。然而，在这些卵母细胞中防止多精入卵并不有效，它们的细胞质中出现了大量精子。这项工作的目的是分析体外成熟的多精入卵的阿根廷蟾蜍卵母细胞中的皮质反应。结果表明，这些卵母细胞的皮质反应似乎按照与该物种产卵卵母细胞所描述的时间顺序发生。此外，在刺破后1分钟，当皮质颗粒发生胞吐作用时，卵母细胞对精子进入变得不敏感，这表明它们能够建立一种对多精入卵的缓慢阻断。
