一种动力学酶直接测量光度计的评估与应用
Evaluation and utilization of a kinetic enzyme direct measuring photometer.
作者信息
Wolf P L
出版信息
J Clin Pathol. 1975 Jul;28(7):587-91. doi: 10.1136/jcp.28.7.587.
We have recently utilized a prototype model of the Beckman Enzyme Activity Analyzer System-TR in our laboratory measuring various serum enzyme activities which include: alkaline phosphatase (ALP), E.C.3.1.3.1; creatine kinase (CK), E.C.2.7.3.2; hydroxybutyrate dehydrogenase (HBD), E.C.1.1.1.30; lactate dehydrogenase (LD), E.C.1.1.1.27; aspartate transaminase (AST), E.C.2.6.1.1; and alanine transaminase (ALT), E.C.2.6.1.2. Precision was found to be good. Sample activities could be measured as high as 1000 IU/1. The carryover studies fell within 2 SD of the means of the enzyme control studies. Coefficients of variation for ALP and CK were in the ranges of 0-40-2-14% and 0-52-4-30%, respectively. Correlation studies were done with GemSAEC and Gilford 300 N Spectrophotometer and the results were accurate, precise, and reproducible.
我们最近在实验室中使用了贝克曼酶活性分析仪系统 -TR 的原型模型来测量各种血清酶活性，这些酶包括：碱性磷酸酶（ALP），酶学委员会编号 3.1.3.1；肌酸激酶（CK），酶学委员会编号 2.7.3.2；羟丁酸脱氢酶（HBD），酶学委员会编号 1.1.1.30；乳酸脱氢酶（LD），酶学委员会编号 1.1.1.27；天冬氨酸转氨酶（AST），酶学委员会编号 2.6.1.1；以及丙氨酸转氨酶（ALT），酶学委员会编号 2.6.1.2。发现精密度良好。样品活性最高可测量到 1000 IU/1。残留研究结果在酶对照研究平均值的 2 个标准差范围内。碱性磷酸酶和肌酸激酶的变异系数分别在 0.40% - 2.14%和 0.52% - 4.30%范围内。使用 GemSAEC 和吉尔福德 300N 分光光度计进行了相关性研究，结果准确、精确且可重复。
Zotero 插件