Ponec M, Gibbs S, Pilgram G, Boelsma E, Koerten H, Bouwstra J, Mommaas M
Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
Skin Pharmacol Appl Skin Physiol. 2001;14 Suppl 1:63-71. doi: 10.1159/000056392.
One of the prerequisites for the use of human skin equivalents for scientific and screening purposes is that their barrier function is similar to that of native skin. Using human epidermis reconstructed on de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged from the extensive production of lamellar bodies, their complete extrusion at the stratum granulosum/SC interface, and the formation of multiple broad lamellar structures in the intercorneocyte space. The presence of well-ordered lipid lamellar phases was confirmed by small-angle X-ray diffraction. Although the long periodicity lamellar phase was present in both the native and the reconstructed epidermis, the short periodicity lamellar phase was present only in native tissue. In addition, the SC lipids predominantly formed the hexagonal sublattice. Analysis of lipid composition revealed that all SC lipids are synthesized in vitro. Differences in SC lipid organization in reconstructed epidermis may be ascribed to the differences in fatty acid content and profile indicating that further improvement in culture conditions is required for generation of in vitro reconstructed epidermis with stratum barrier properties of the native tissue.
将人类皮肤替代物用于科学研究和筛选目的的前提条件之一是其屏障功能与天然皮肤相似。利用在去表皮真皮上重建的人类表皮，我们证明，从板层小体的大量产生、它们在颗粒层/角质层界面的完全挤出以及角质形成细胞间空间中多个宽阔板层结构的形成来看，角质层（SC）屏障在体外的形成过程与体内相似。小角X射线衍射证实了有序脂质板层相的存在。虽然长周期板层相在天然表皮和重建表皮中均存在，但短周期板层相仅存在于天然组织中。此外，角质层脂质主要形成六方亚晶格。脂质组成分析表明，所有角质层脂质均在体外合成。重建表皮中角质层脂质组织的差异可能归因于脂肪酸含量和分布的差异，这表明需要进一步改善培养条件，以生成具有天然组织角质层屏障特性的体外重建表皮。
