Douglas T C, Dowsett A P
J Immunol. 1975 Jul;115(1):283-8.
AKR/Cum mice (Thy-1b = thetaC3H) immunized with nucleated cells from WF rat thymus, Peyer's patches, peritoneal exudate, mesenteric lymph nodes, blood, bone marrow, or spleen produced antibodies cytotoxic for ADR/J (Thy-1a = thetaAKR) but not for AKR/Cum thymocytes. The specificity of these antibodies for the Thy-1.1 (theta-AKR) antigen was confirmed by tests using thymocytes from backcross mice segregating at the Thy-1 locus. This result suggested that the rat lymphocyte antgen cross-reactive with Thy-1.1 was expressed by at least some members of each of the rat lymphoid cell populations tested. AKR/Cum mice immunized with killed rat cells also produced anti-Thy-1.1 antibodies; thus indicating that further differentiation of the injected cells was not a prerequisite for the anti-Thy-1.1 response. Unexpectedly, about 9% of unimmunized adult AKR/Cum males were found to be producing antibodies against Thy-1.1. To our knowledge, natural antibodies of this specificity have not been previously reported. Finally, it was found that peritoneal exudate cells taken from WF rats previously immunized with EL-4 mouse leukemia cells were neither killed nor functionally inactivated by treatment with anti-Thy-1.1 antibodies and complement.
用来自WF大鼠胸腺、派伊尔氏结、腹腔渗出液、肠系膜淋巴结、血液、骨髓或脾脏的有核细胞免疫的AKR/Cum小鼠（Thy-1b = thetaC3H）产生了对ADR/J（Thy-1a = thetaAKR）具有细胞毒性的抗体，但对AKR/Cum胸腺细胞没有细胞毒性。使用在Thy-1位点分离的回交小鼠的胸腺细胞进行的测试证实了这些抗体对Thy-1.1（theta-AKR）抗原的特异性。这一结果表明，与Thy-1.1交叉反应的大鼠淋巴细胞抗原在每个测试的大鼠淋巴细胞群体的至少一些成员中表达。用杀死的大鼠细胞免疫的AKR/Cum小鼠也产生了抗Thy-1.1抗体；因此表明注入细胞的进一步分化不是抗Thy-1.1反应的先决条件。出乎意料的是，发现约9%未免疫的成年AKR/Cum雄性小鼠产生针对Thy-1.1的抗体。据我们所知，这种特异性的天然抗体以前尚未见报道。最后，发现从先前用EL-4小鼠白血病细胞免疫的WF大鼠获取的腹腔渗出细胞在用抗Thy-1.1抗体和补体处理后既未被杀死也未在功能上失活。
