Zhou J, Allred D C, Avis I, Martínez A, Vos M D, Smith L, Treston A M, Mulshine J L
Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Breast Cancer Res Treat. 2001 Apr;66(3):217-24. doi: 10.1023/a:1010631915831.
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP-A2/B1) is highly expressed during critical stages of lung development and carcinogenesis. To determine if the expression of hnRNP-A2/B1 is an informative biomarker in breast carcinogenesis, we analyzed hnRNP-A2/B1 overexpression by immunohistochemistry in archived specimens. Expression was detected in 48/85 (56.5%) primary invasive breast cancers and 7/72 (9.7%) specimens of normal breast tissue. Northern analysis of breast cancer cells also demonstrated higher level of hnRNP-A2/B1 expression compared to normal or transformed breast cells. Expression of hnRNP-A2/B1 in breast cancer cells was decreased by exposure to retinoids coordinately with decreased cell growth. These results warrant further evaluation of hnRNP-A2/B1 as a marker of breast carcinogenesis.
异质性核核糖核蛋白A2/B1（hnRNP - A2/B1）在肺发育和致癌作用的关键阶段高度表达。为了确定hnRNP - A2/B1的表达是否为乳腺癌发生中的一种信息性生物标志物，我们通过免疫组织化学分析了存档标本中hnRNP - A2/B1的过表达情况。在48/85（56.5%）的原发性浸润性乳腺癌和7/72（9.7%）的正常乳腺组织标本中检测到了表达。对乳腺癌细胞的Northern分析也表明，与正常或转化的乳腺细胞相比，hnRNP - A2/B1的表达水平更高。暴露于视黄酸会使乳腺癌细胞中hnRNP - A2/B1的表达降低，同时细胞生长也会减少。这些结果值得对hnRNP - A2/B1作为乳腺癌发生标志物进行进一步评估。
