Hinteregger C, Streichsbier F
Section of Environmental Microbiology, Institute of Biochemical Technology and Microbiology, University of Technology Vienna, Getreidemarkt 9, A-1060 Vienna, Austria.
J Basic Microbiol. 2001;41(3-4):159-70. doi: 10.1002/1521-4028(200107)41:3/4<159::aid-jobm159>3.0.co;2-p.
A bacterial strain capable of utilizing toluidine isomers as its sole source of carbon and energy for growth was isolated from contaminated soil. The isolate was identified as Achromobacter xylosoxidans and was designated strain T7. Strain T7 differs from other toluidine-degrading strains with respect to the use of all three toluidine isomers even as an equimolar mixture. Additionally, strain T7 harbours the ability to use aniline, phenol, and cresols as growth substrates. Utilization of the toluidine isomers was demonstrated by an increase in the bacterial biomass concomitant with a decrease of the respective toluidine concentration in liquid medium with this compound as sole source of carbon and energy. No accumulation of any intermediate was detectable by HPLC-analyses. Results of oxygen uptake experiments with resting cells of strain T7 pre-grown on the respective toluidine and enzymatic investigations in cell-free extracts indicate the metabolization of the toluidines via the respective methylcatechols as intermediates. These compounds are substrates for the meta-cleavage pathway initiated by inducible catechol 2,3-dioxygenase found in toluidine-grown cells of strain T7.
从受污染土壤中分离出一种能够利用甲苯胺异构体作为其唯一碳源和能源进行生长的细菌菌株。该分离株被鉴定为木糖氧化无色杆菌，并命名为T7菌株。T7菌株与其他甲苯胺降解菌株的不同之处在于，它能够利用所有三种甲苯胺异构体，即使是以等摩尔混合物的形式。此外，T7菌株具有利用苯胺、苯酚和甲酚作为生长底物的能力。以甲苯胺异构体作为唯一碳源和能源的液体培养基中，细菌生物量增加，相应甲苯胺浓度降低，证明了对甲苯胺异构体的利用。通过HPLC分析未检测到任何中间产物的积累。用在相应甲苯胺上预培养的T7菌株静息细胞进行的氧摄取实验结果以及无细胞提取物中的酶学研究表明，甲苯胺通过相应的甲基邻苯二酚作为中间产物进行代谢。这些化合物是由在T7菌株甲苯胺生长细胞中发现的可诱导邻苯二酚2,3-双加氧酶启动的间位裂解途径的底物。
