Dauphin G, Legay V, Sailleau C, Smondack S, Hammoumi S, Zientara S
AFSSA Alfort, 22 rue Pierre Curie, 94703 Maisons-Alfort cedex, France1.
J Gen Virol. 2001 Sep;82(Pt 9):2199-2204. doi: 10.1099/0022-1317-82-9-2199.
Borna disease virus (BDV) is an enveloped, non-segmented negative-stranded RNA virus which belongs to the Bornaviridae family. BDV is an aetiological agent of encephalitis in horses, sheep and several other vertebrate species. In order to extend our knowledge about the presence of BDV in France, a study based on BDV RNA detection by RT-nested-PCR was done with 196 animal tissues: 171 brain samples collected from different animal species (75 horses, 59 foxes, 31 cattle, 4 dogs, 1 sheep, 1 roe deer) and 25 horse blood samples. An RNA internal standard molecule was constructed and was co-amplified with the test template. This study reports the first detection of BDV RNA in France in 10 brain samples collected from horses, foxes and cattle, and from 14 horse blood samples. Detection of the BDV genome in the brains of six red foxes is the first evidence of BDV infection in this species.
博尔纳病病毒（BDV）是一种有包膜的、不分节段的负链RNA病毒，属于博尔纳病毒科。BDV是马、绵羊和其他几种脊椎动物脑炎的病原体。为了扩展我们对法国BDV存在情况的了解，我们对196份动物组织进行了一项基于逆转录巢式聚合酶链反应（RT-nested-PCR）检测BDV RNA的研究：从不同动物物种收集的171份脑样本（75匹马、59只狐狸、31头牛、4只狗、1只绵羊、1只狍）和25份马血样本。构建了一个RNA内标分子，并与测试模板共同扩增。本研究报告了在法国首次从10份来自马、狐狸和牛的脑样本以及14份马血样本中检测到BDV RNA。在六只赤狐的脑中检测到BDV基因组是该物种感染BDV的首个证据。
