Bina M, Crowely E
Department of Chemistry, Purdue University, W. Lafayette, IN 47907-1393, USA.
Biopolymers. 2001 Oct 15;59(5):347-55. doi: 10.1002/1097-0282(20011015)59:5<347::AID-BIP1032>3.0.CO;2-6.
In studies of both short and relatively long human genomic DNA, we found a clustering of the consensus site for the transcription factor GCF at the 5' boundary of a subset of human genes. In studies of promoter regions with known transcription initiation site, the cluster of consensus GCF site appeared near the transcription initiation site and in some sequences it extended into the transcribed region defining the leader mRNA. We also found a detectable correlation between the 5' boundary of human genes and recognition motifs for other transcription factors that bind to GC-rich sequences. But in these cases, the correlation was not as general as the correlation observed for the consensus GCF site.
在对人类短基因组DNA和相对较长基因组DNA的研究中，我们发现在一部分人类基因的5'边界处，转录因子GCF的共有位点出现了聚集现象。在对具有已知转录起始位点的启动子区域的研究中，共有GCF位点的聚集出现在转录起始位点附近，并且在一些序列中延伸到转录区域，界定了前导mRNA。我们还发现人类基因的5'边界与其他结合富含GC序列的转录因子的识别基序之间存在可检测到的相关性。但在这些情况下，这种相关性不像在共有GCF位点观察到的相关性那样普遍。
