Hurley M J, Gerard D J, Jauniaux E, Stern G M, Uchida K, Bradford H F
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom.
J Neural Transm (Vienna). 2001;108(7):781-92. doi: 10.1007/s007020170028.
Obtaining an adequate supply of foetal dopaminergic tissue to treat Parkinson's disease by neural transplantation can be difficult. In this study primary cultures of human foetal cerebral cortex cells were transfected, using cationic lipids, with a eukaryotic expression vector (pCIneo-THI) containing the cDNA for human tyrosine hydroxylase isoform I (TH). Cortical cells from human (10-14 week) foetuses were cultured for 11 days in vitro and transfected twice with pCIneo-THI during this time. The double transfection process resulted in 3-4% of the cells becoming TH positive. When grafted into the striatum of 6-OHDA lesioned rats the transfected foetal cerebral cortex cells reduced amphetamine-induced circling behaviour by 75%, while grafts of untransfected cells had no significant effect on turning. TH transfected foetal cerebral cortex cells may therefore be a useful alternative supply of tissue for use in neural transplants to treat Parkinson's disease.
通过神经移植获得足够的胎儿多巴胺能组织来治疗帕金森病可能会很困难。在本研究中，使用阳离子脂质将人胎儿大脑皮层细胞的原代培养物用含有人类酪氨酸羟化酶同工型I（TH）cDNA的真核表达载体（pCIneo-THI）进行转染。来自人（10 - 14周）胎儿的皮层细胞在体外培养11天，并在此期间用pCIneo-THI转染两次。双重转染过程导致3 - 4%的细胞变为TH阳性。当移植到6-OHDA损伤大鼠的纹状体中时，转染的胎儿大脑皮层细胞使苯丙胺诱导的转圈行为减少了75%，而未转染细胞的移植对转圈行为没有显著影响。因此，TH转染的胎儿大脑皮层细胞可能是用于神经移植治疗帕金森病的一种有用的替代组织来源。
