Theoretical considerations on mononuclear aggregations of mixed nucleosides in dilute aqueous solutions based upon osmometric measurements.

A theory is presented based on experimental work reporting shifts in the critical micelle-like concentrations of nucleosides and caffeine. An increase in these concentrations by multiple integers (called complexing capacity numbers) of the concentration of the vehicle compound, whose concentration was fixed, was observed for substances that tend to form complexes with each other and with some other compounds and to self-associate as well when their concentrations are varied. The physical model presented assumes the significance of the potential hydrogen bonding sites in determining the mode of aggregation as well as the magnitude of these complexing capacity numbers. This assumption was verified experimentally when reducing the hydrogen bonding by using 1,3-dimethyluracil. The mathematical model hypothesized has provided a means for calculating equilibrium constants for various consecutive processes as well as other quantities of theoretical interest.