Matousek M, Carati C, Gannon B, Mitsube K, Brännström M
Department of Obstetrics and Gynaecology, Göteborg University, Sahlgrenska University Hospital, S-413 45, Göteborg, Sweden.
Eur J Obstet Gynecol Reprod Biol. 2001 Sep;98(1):46-52. doi: 10.1016/s0301-2115(01)00338-4.
ovulation is associated with degradation of the follicular apex vasodilatation and increased permeability of ovarian vessels. These changes may maintain or increase intrafollicular pressure (IFP) at ovulation to cause rupture of the follicular wall.
to investigate the possible regulation of IFP during the ovulatory process.
immature Sprague-Dawley rats were primed with pregnant mare serum gonadotrophin (PMSG; 10IU) and given hCG (10IU) 48h later. The ovary was exposed 48-60h after PMSG, micropipette inserted into the Graafian follicle and the IFP measured at three time periods: preovulatory (PO) 48h after PMSG; midovulatory (MO) 4-7h after hCG; late ovulatory (LO) 9-12h after hCG. The offset of the nitric oxide synthase (NOS) inhibitor L-arginine methyl ester (L-NAME), the alpha(1)-adrenoceptor agonist phenylephrine and the beta-adrenoceptor agonist isoprenaline were tested.
phenylephrine given i.v. increased the systemic blood pressure, and significantly decreased the IFP in the LO phase (78% of pre-treatment value). Local administration of phenylephrine or isoprenaline (1ml of 1.5-15 microM) by superfusion over the ovary did not change the IFP. Local administration of L-NAME (1ml of 2 microM) significantly lowered (P<0.05) the IFP in the MO and LO phases, but was without effect in the PO phase.
this study reveals that IFP regulation may be related to changes of the systemic blood pressure and that NO may be one local ovarian mediator in IFP regulation.
排卵与卵泡顶端血管舒张的退化以及卵巢血管通透性增加有关。这些变化可能在排卵时维持或增加卵泡内压（IFP），从而导致卵泡壁破裂。
研究排卵过程中卵泡内压可能的调节机制。
对未成熟的斯普拉格-道利大鼠注射孕马血清促性腺激素（PMSG；10IU）进行预处理，并在48小时后给予人绒毛膜促性腺激素（hCG；10IU）。在注射PMSG后48 - 60小时暴露卵巢，将微量移液器插入格拉夫卵泡，并在三个时间段测量卵泡内压：排卵前（PO），注射PMSG后48小时；排卵中期（MO），注射hCG后4 - 7小时；排卵后期（LO），注射hCG后9 - 12小时。测试一氧化氮合酶（NOS）抑制剂L - 精氨酸甲酯（L - NAME）、α₁ - 肾上腺素能受体激动剂去氧肾上腺素和β - 肾上腺素能受体激动剂异丙肾上腺素的作用。
静脉注射去氧肾上腺素可升高全身血压，并显著降低排卵后期的卵泡内压（降至治疗前值的78%）。通过卵巢灌注局部给予去氧肾上腺素或异丙肾上腺素（1ml，1.5 - 15μM）未改变卵泡内压。局部给予L - NAME（1ml，2μM）可显著降低（P < 0.05）排卵中期和后期的卵泡内压，但对排卵前期无影响。
本研究表明，卵泡内压的调节可能与全身血压变化有关，一氧化氮可能是卵泡内压调节中的一种局部卵巢介质。
