West C M, Davidson S E, Elyan S A, Valentine H, Roberts S A, Swindell R, Hunter R D
CRC Experimental Radiation Oncology Group, Paterson Institute for Cancer Research, Manchester, United Kingdom.
Int J Radiat Oncol Biol Phys. 2001 Sep 1;51(1):10-5. doi: 10.1016/s0360-3016(01)01575-9.
To study the relationship between pretreatment peripheral blood lymphocyte radiosensitivity and morbidity following radiation therapy.
A prospective study was carried out in which patients with carcinoma of the cervix underwent radiation therapy. Intrinsic radiosensitivity was measured on pretreatment peripheral blood lymphocytes, using a limiting dilution clonogenic assay. Late morbidity was assessed using the Franco-Italian glossary. Results were correlated in an actuarial analysis.
There were no correlations between the measured lymphocyte radiosensitivity (SF2) and colony-forming efficiency, patient age, tumor grade, or disease stage. For 83 patients, lymphocyte SF2 was a significant prognostic factor for the probability of developing both any (p = 0.002) and Grade 3 (p = 0.026) morbidity. In 174 patients, stage showed borderline significance as a prognostic factor for morbidity (p = 0.056). However, the type of treatment (intracavitary alone, intracavitary plus parametrial irradiation, single insertion plus whole-pelvis irradiation) was significantly associated with the probability of developing late complications (p = 0.013). There was a weak significant inverse correlation between lymphocyte SF2 and grade of morbidity (r = -0.34, p = 0.002).
These data highlight the importance of normal cell radiosensitivity as a factor determining radiation therapy response. They also show that peripheral blood lymphocyte SF2 is a highly significant prognostic factor for the probability of developing late radiation morbidity, and that carcinoma of the cervix is a good model for testing radiobiologic principles in the clinic.
研究放疗前外周血淋巴细胞放射敏感性与放疗后发病率之间的关系。
对宫颈癌患者进行放疗的前瞻性研究。采用极限稀释克隆形成试验测定放疗前外周血淋巴细胞的内在放射敏感性。使用法意词汇表评估晚期发病率。在精算分析中对结果进行相关性分析。
所测淋巴细胞放射敏感性（SF2）与集落形成效率、患者年龄、肿瘤分级或疾病分期之间无相关性。对于83例患者，淋巴细胞SF2是发生任何（p = 0.002）和3级（p = 0.026）并发症概率的显著预后因素。在174例患者中，分期作为并发症的预后因素具有临界显著性（p = 0.056）。然而，治疗类型（单纯腔内放疗、腔内加宫旁照射、单次插植加全盆腔照射）与发生晚期并发症的概率显著相关（p = 0.013）。淋巴细胞SF2与并发症分级之间存在弱显著负相关（r = -0.34，p = 0.002）。
这些数据突出了正常细胞放射敏感性作为决定放疗反应因素的重要性。它们还表明外周血淋巴细胞SF2是发生晚期放射并发症概率的高度显著预后因素，并且宫颈癌是在临床中测试放射生物学原理的良好模型。
