Arroyo J, Miller C A, Catalan J, Monath T P
Acambis Inc. 38 Sidney Street, Cambridge, MA 02319, USA.
Trends Mol Med. 2001 Aug;7(8):350-4. doi: 10.1016/s1471-4914(01)02048-2.
By combining molecular-biological techniques with our increased understanding of the effect of gene sequence modification on viral function, yellow fever 17D, a positive-strand RNA virus vaccine, has been manipulated to induce a protective immune response against viruses of the same family (e.g. Japanese encephalitis and dengue viruses). Triggered by the emergence of West Nile virus infections in the New World afflicting humans, horses and birds, the success of this recombinant technology has prompted the rapid development of a live-virus attenuated candidate vaccine against West Nile virus.
通过将分子生物学技术与我们对基因序列修饰对病毒功能影响的深入理解相结合，黄热病17D（一种正链RNA病毒疫苗）已被改造，以诱导针对同一家族病毒（如日本脑炎病毒和登革病毒）的保护性免疫反应。受西尼罗河病毒在新世界感染人类、马匹和鸟类的出现所触发，这种重组技术的成功促使了一种针对西尼罗河病毒的减毒活病毒候选疫苗的快速研发。
