Aronsson F, Karlsson H, Ljunggren H G, Kristensson K
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
J Neurovirol. 2001 Apr;7(2):117-24. doi: 10.1080/13550280152058771.
Strains of influenza A virus are known to infect specific subpopulations of neurons in the mouse brain. Here we report that all segments of the genome of the neurotropic influenza A virus, strain WSN/33, can persist in the brains of immunodefective transporter associated with Antigen Processing 1 (TAP1) mutant mice. Ten to 17 months after injection of virus into the olfactory bulbs, viral RNA encoding the nonstructural NS1 protein was detected in sections from the brain at midbrain levels by RT-PCR in almost all animals. Both negative-strand genomic RNA (vRNA) and positive-strand RNA, including mRNA, were found. RNA encoding nucleoprotein and polymerases, which form the replicative complex of the virus, were detected in fewer brains. RNA encoding envelope proteins were found only in occasional brains. No viral cDNA could be identified. This observation shows that certain regions of the brain in immunodefective mice may harbor the genome of influenza A virus including the NS1 gene, the products of which may play a regulatory role in host-cell metabolism.
已知甲型流感病毒毒株可感染小鼠大脑中的特定神经元亚群。在此，我们报告嗜神经性甲型流感病毒WSN/33株的基因组所有片段均可在与抗原加工相关的转运体1（TAP1）突变免疫缺陷小鼠的大脑中持续存在。在将病毒注射到嗅球10至17个月后，通过逆转录聚合酶链反应（RT-PCR）在几乎所有动物中脑水平的脑切片中检测到了编码非结构NS1蛋白的病毒RNA。同时发现了负链基因组RNA（vRNA）和正链RNA，包括信使核糖核酸（mRNA）。在较少的大脑中检测到了编码核蛋白和聚合酶的RNA，它们构成病毒的复制复合体。仅在偶尔的大脑中发现了编码包膜蛋白的RNA。未鉴定出病毒互补脱氧核糖核酸（cDNA）。这一观察结果表明，免疫缺陷小鼠大脑的某些区域可能含有甲型流感病毒的基因组，包括NS1基因，其产物可能在宿主细胞代谢中发挥调节作用。
