Gerasimov I G, Ignatov D Iu
Research Institute of Family Problems, Donetsk Medical University, 83048 Donetsk.
Tsitologiia. 2001;43(5):432-6.
Kinetics of neutrophil inactivation was investigated in vitro by Nitroblue tetrazolium (NBT) test in the process of their contact with the substrate. It has been shown that the previous thermostatation results in an exclusive inactivation of neutrophils with high reaction ability leading to their complete inactivation. Such an inactivation is a consequence of cell contacts with the substrate, whose chemical structure and physicochemical properties define the process regularities. The neutrophil inactivation is probably not a consequence of the contact itself but may follow the next scheme: stimulus (contact with substrate)--generation of reactive oxygen metabolites--inactivation. Two functional unequal classes of neutrophils were differentiated on the basis of different levels of their reactive oxygen metabolite generation, and on their ability to inactivation. In vitro cells of one of these classes actively generate reactive oxygen metabolites to be inactivated in consequence of interaction with the substrate, whereas cells of the other class produce reactive oxygen metabolites less actively and are nor inactivated. Evidently, in vivo cells of the are phagocytes and those of the latter fulfill other functions.
通过在体外利用硝基蓝四氮唑（NBT）试验研究中性粒细胞与底物接触过程中的失活动力学。结果表明，预先进行恒温处理会导致具有高反应能力的中性粒细胞完全失活。这种失活是细胞与底物接触的结果，底物的化学结构和物理化学性质决定了该过程的规律。中性粒细胞的失活可能不是接触本身的结果，而是可能遵循以下模式：刺激（与底物接触）——活性氧代谢产物的产生——失活。根据活性氧代谢产物产生水平的不同以及失活能力的差异，区分出了两类功能不同的中性粒细胞。在体外，其中一类细胞会积极产生活性氧代谢产物，因与底物相互作用而失活，而另一类细胞产生活性氧代谢产物的活性较低且不会失活。显然，在体内，前者的细胞是吞噬细胞，而后者的细胞履行其他功能。
