Ljungmann K, Hartmann B, Kissmeyer-Nielsen P, Flyvbjerg A, Holst J J, Laurberg S
Surgical Research Unit, Department of Surgery L, Aarhus University Hospital, DK-8000 Aarhus C, Denmark.
Am J Physiol Gastrointest Liver Physiol. 2001 Sep;281(3):G779-85. doi: 10.1152/ajpgi.2001.281.3.G779.
Existing data on morphological adaptation after small bowel resection are obtained by potentially biased methods. Using stereological techniques, we examined segments of bowel on days 0, 4, 7, 14, and 28 after 80% jejunoileal resection or sham operation in rats and correlated intestinal growth with plasma levels of glucagon-like peptide-2 (GLP-2). In the jejunum and ileum of the resected rats, the mucosal weight increased by 120 and 115% during the first week, and the weight of muscular layer increased by 134 and 83%, compared with sham-operated controls. The luminal surface area increased by 190% in the jejunum and by 155% in the ileum after 28 days. The GLP-2 level was increased by 130% during the entire study period in the resected rats. Small bowel resection caused a pronounced and persistent transmural growth response in the remaining small bowel, with the most prominent growth occurring in the jejunal part. The significantly elevated GLP-2 level is consistent with an important role of GLP-2 in the adaptive response.
目前关于小肠切除术后形态学适应的现有数据是通过可能存在偏差的方法获得的。我们使用体视学技术，在大鼠进行80%空肠回肠切除或假手术后的第0、4、7、14和28天检查肠段，并将肠道生长与胰高血糖素样肽-2（GLP-2）的血浆水平相关联。与假手术对照组相比，切除术后大鼠的空肠和回肠黏膜重量在第一周分别增加了120%和115%，肌层重量分别增加了134%和83%。28天后，空肠腔表面积增加了190%，回肠增加了155%。在整个研究期间，切除术后大鼠的GLP-2水平升高了130%。小肠切除导致剩余小肠出现明显且持续的全层生长反应，最显著的生长发生在空肠部分。GLP-2水平的显著升高与GLP-2在适应性反应中的重要作用一致。
