Kaczmarski K, Antos D, Sajonz H, Sajonz P, Guiochon G
Department of Chemistry, The University of Tennessee, Knoxville 37996-1600, USA.
J Chromatogr A. 2001 Aug 3;925(1-2):1-17. doi: 10.1016/s0021-9673(01)01035-4.
The experimental results of a previous study of the mass transfer kinetics of bovine serum albumin (BSA) in ion-exchange chromatography, under nonlinear conditions, were reevaluated using the general rate model of chromatography. Solutions of this model were obtained numerically. The influences of axial dispersion, the resistance to mass transfer from the bulk mobile phase to the surface of the packing particles, and the intraparticle mass transfer resistances on the profiles of the breakthrough curves of BSA were investigated. The results obtained are compared to those of a previous investigation of the same data, using the simple transport-dispersive model and the lumped pore diffusion model. The results obtained show that the use of an oversimplified model for the analysis of chromatographic data can lead to erroneous interpretations of the experimental data and to misunderstandings of the fundamentals of the processes involved. Finally, a theoretical comparison between the properties and the range of application of the three models is provided.
利用色谱通用速率模型对先前一项关于牛血清白蛋白（BSA）在离子交换色谱中非线性条件下传质动力学的实验结果进行了重新评估。该模型的解通过数值方法获得。研究了轴向扩散、从主体流动相到填充颗粒表面的传质阻力以及颗粒内传质阻力对BSA穿透曲线轮廓的影响。将所得结果与先前使用简单传输-扩散模型和集总孔扩散模型对相同数据进行研究的结果进行了比较。所得结果表明，使用过于简化的模型分析色谱数据可能会导致对实验数据的错误解释以及对所涉及过程基本原理的误解。最后，对这三种模型的性质和应用范围进行了理论比较。
