Kan T, Shimada Y, Sato F, Maeda M, Kawabe A, Kaganoi J, Itami A, Yamasaki S, Imamura M
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Kawaracho 54, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Biochem Biophys Res Commun. 2001 Aug 31;286(4):792-801. doi: 10.1006/bbrc.2001.5400.
Human esophageal cancer cell lines and human esophageal cancer tissues were profiled on cDNA microarrays. In esophageal cancer cell lines, KYAE and OE-33 (adenocarcinomas) were distinguished from KYSE series (squamous cell carcinomas). Although SK-GT-4 and TE7 were derived from adenocarcinomas, they had a comparatively similar expression profile to the KYSE series. A set of genes whose expression commonly either increased or decreased in cancer cell lines was identified. Genes that were characteristically expressed in KYAE and OE-33 were also identified. The gene expression profiles of cancer tissues (CTs) were remarkably different from those of the cancer cell lines (CCLs). Notable differences between CCLs and CTs were observed in matrix metalloproteinases, plasminogen activator, collagens, paxillin, and thrombospondin 2, etc., whose expression was not increased in CCLs but increased in CTs. Twenty-three genes were extracted to categorize patients according to their prognoses, and clustering analyses, using these genes, were performed successfully.
在cDNA微阵列上对人食管癌细胞系和人食管癌组织进行了分析。在食管癌细胞系中，KYAE和OE - 33（腺癌）与KYSE系列（鳞状细胞癌）不同。尽管SK - GT - 4和TE7源自腺癌，但它们具有与KYSE系列相对相似的表达谱。确定了一组在癌细胞系中表达普遍增加或减少的基因。还确定了在KYAE和OE - 33中特异性表达的基因。癌组织（CTs）的基因表达谱与癌细胞系（CCLs）的显著不同。在基质金属蛋白酶、纤溶酶原激活剂、胶原蛋白、桩蛋白和血小板反应蛋白2等方面观察到CCLs和CTs之间的显著差异，其在CCLs中表达未增加，但在CTs中增加。提取了23个基因以根据患者的预后进行分类，并成功地使用这些基因进行了聚类分析。
