Sanchez-Ramos J R, Song S, Kamath S G, Zigova T, Willing A, Cardozo-Pelaez F, Stedeford T, Chopp M, Sanberg P R
Center for Aging and Brain Repair, Department of Neurology, University of South Florida College of Medicine, Tampa, Florida, USA.
Exp Neurol. 2001 Sep;171(1):109-15. doi: 10.1006/exnr.2001.7748.
A population of cells derived from human and rodent bone marrow has been shown by several groups of investigators to give rise to glia and neuron-like cells. Here we show that human umbilical cord blood cells treated with retinoic acid (RA) and nerve growth factor (NGF) exhibited a change in phenotype and expressed molecular markers usually associated with neurons and glia. Musashi-1 and beta-tubulin III, proteins found in early neuronal development, were expressed in the induced cord blood cells. Other molecules associated with neurons in the literature, such as glypican 4 and pleiotrophin mRNA, were detected using DNA microarray analysis and confirmed independently with reverse transcriptase polymerase chain reaction (RT-PCR). Glial fibrillary acidic protein (GFAP) and its mRNA were also detected in both the induced and untreated cord blood cells. Umbilical cord blood appears to be more versatile than previously known and may have therapeutic potential for neuronal replacement or gene delivery in neurodegenerative diseases, trauma, and genetic disorders.
几组研究人员已表明，源自人类和啮齿动物骨髓的细胞群能够产生神经胶质细胞和神经元样细胞。在此我们表明，用视黄酸（RA）和神经生长因子（NGF）处理的人脐带血细胞表现出表型变化，并表达通常与神经元和神经胶质细胞相关的分子标记。在早期神经元发育中发现的蛋白质Musashi-1和β-微管蛋白III在诱导的脐带血细胞中表达。使用DNA微阵列分析检测到文献中与神经元相关的其他分子，如磷脂酰肌醇蛋白聚糖4和多效生长因子mRNA，并通过逆转录聚合酶链反应（RT-PCR）独立证实。在诱导和未处理的脐带血细胞中也检测到胶质纤维酸性蛋白（GFAP）及其mRNA。脐带血似乎比以前认为的更具通用性，可能对神经退行性疾病、创伤和遗传疾病中的神经元替代或基因传递具有治疗潜力。
