Chaudhuri T R, Mountz J M, Rogers B E, Partridge E E, Zinn K R
Department of Radiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
Gynecol Oncol. 2001 Sep;82(3):581-9. doi: 10.1006/gyno.2001.6297.
The purpose of the study was to develop a sensitive, noninvasive imaging method for monitoring ovarian xenografts during therapeutic intervention.
Human ovarian tumor cells (SKOV3.ip1) were infected with a replication-deficient adenoviral (Ad) vector encoding green fluorescent protein (GFP). The GFP-positive tumor cells were imaged in vitro and in vivo with a fluorescence stereomicroscope. Using appropriate filters, both GFP fluorescence and adriamycin were simultaneously detected. Nude mice implanted with GFP-positive cells were imaged repeatedly, in a noninvasive manner.
SKOV3.ip1 cells infected with Ad-GFP showed high GFP fluorescence, which was eliminated after treatment with adriamycin. Loss of GFP fluorescence was confirmed to be dead cells. For in vivo imaging, intraperitoneal tumors as small as 0.2 mm in diameter were detected externally. Adriamycin uptake was detected in tumors by in vivo imaging, and reduction in tumor size was concurrent with decrease in GFP fluorescence. These findings were confirmed at necropsy.
Fluorescence stereomicroscopy monitored the response of ovarian xenografts to adriamycin therapy. For the first time, GFP and adriamycin were imaged simultaneously.
本研究的目的是开发一种灵敏的非侵入性成像方法，用于在治疗干预期间监测卵巢异种移植瘤。
用人卵巢肿瘤细胞（SKOV3.ip1）感染编码绿色荧光蛋白（GFP）的复制缺陷型腺病毒（Ad）载体。用荧光立体显微镜对GFP阳性肿瘤细胞进行体外和体内成像。使用合适的滤光片，同时检测GFP荧光和阿霉素。对植入GFP阳性细胞的裸鼠进行重复的非侵入性成像。
感染Ad-GFP的SKOV3.ip1细胞显示出高GFP荧光，在用阿霉素处理后荧光消失。GFP荧光的消失被证实为细胞死亡。对于体内成像，能从外部检测到直径小至0.2毫米的腹腔肿瘤。通过体内成像在肿瘤中检测到阿霉素摄取，并且肿瘤大小的减小与GFP荧光的降低同时发生。这些发现尸检时得到证实。
荧光立体显微镜监测了卵巢异种移植瘤对阿霉素治疗的反应。首次同时对GFP和阿霉素进行成像。
