Itagaki T, Honnami M, Ito D, Ito K, Tsutsumi K, Terasaki K, Shibahara T, Noda Y
Department of Parasitology, Iwate University, Morioka, Japan.
J Helminthol. 2001 Jun;75(2):193-6.
Mitochondrial DNA polymorphism was characterized in a triploid form of Fasciola found in Japan in comparison with F. hepatica, F. gigantica and Korean Fasciola worm. Seventy worms of Fasciola from Japan, three of F. hepatica from Uruguay and Australia, two of F. gigantica from Thailand and one of Fasciola from Korea were used in the study. Mitochondrial DNA polymorphism was detected by restriction fragment length polymorphism (RFLP) using eight restriction enzymes, BamH I, Bgl II, Dra I, EcoR I, EcoR V, Hind III, Mfl I and Sca I. Three different types (types 1, 2 and 3) were detected from 76 Fasciola worms used in the study. Eight of 70 Japanese worms were categorized in type 2 (F. gigantica type), and the remaining 62 were in type 3 (F. hepatica type).
对在日本发现的三倍体肝片吸虫的线粒体DNA多态性进行了表征，并与肝片吸虫、巨片吸虫和韩国肝片吸虫进行了比较。该研究使用了来自日本的70条肝片吸虫、来自乌拉圭和澳大利亚的3条肝片吸虫、来自泰国的2条巨片吸虫以及来自韩国的1条肝片吸虫。使用BamH I、Bgl II、Dra I、EcoR I、EcoR V、Hind III、Mfl I和Sca I这8种限制性内切酶，通过限制性片段长度多态性（RFLP）检测线粒体DNA多态性。在该研究使用的76条肝片吸虫中检测到三种不同类型（类型1、2和3）。70条日本肝片吸虫中有8条被归类为类型2（巨片吸虫类型），其余62条为类型3（肝片吸虫类型）。
