Reidy B, Nösberger J, Fleming A
Institute of Plant Sciences, Swiss Federal Institute of Technology (ETH) Zürich, Universitätsstrasse 2, 8092 Zürich, Switzerland.
J Exp Bot. 2001 Sep;52(362):1847-56. doi: 10.1093/jexbot/52.362.1847.
Festuca pratensis Huds. is a forage grass with the ability to withstand harsh climatic conditions. However, its potential agronomic use is limited by its poor competitive ability, which can be traced to limitations in leaf growth. In order to characterize this process and to identify genes which might function as markers for leaf growth, three XET-related genes in the leaf elongation zone (LEZ) of F. pratensis are reported. A detailed expression analysis is presented of the three genes in two F. pratensis genotypes with contrasting leaf growth characteristics grown under two nitrogen levels. By means of a detailed spatial analysis of growth and XET encoding transcript pattern along the LEZ, a specific correlation is shown between FpXET1 expression and tissue elongation that is maintained under the different growth conditions, while the two other XETs expressed in the LEZ show different transcript dynamics. Tissue localization of FpXET1 and FpXET2 transcripts indicate an accumulation throughout young tissue, which is consistent with the encoded proteins playing roles in cell wall modification processes during growth. It is proposed that FpXET1 is a potential marker for tissue elongation and leaf growth in F. pratensis.
高羊茅是一种能够耐受恶劣气候条件的饲草。然而，其潜在的农艺用途受到其竞争力差的限制，这可追溯到叶片生长的局限性。为了表征这一过程并鉴定可能作为叶片生长标记的基因，本文报道了高羊茅叶片伸长区（LEZ）中的三个与木葡聚糖内转糖基酶（XET）相关的基因。对在两种氮水平下生长的具有不同叶片生长特性的两种高羊茅基因型中的这三个基因进行了详细的表达分析。通过对沿LEZ的生长和XET编码转录本模式进行详细的空间分析，发现FpXET1表达与组织伸长之间存在特定的相关性，且在不同生长条件下保持不变，而在LEZ中表达的另外两个XET显示出不同的转录动态。FpXET1和FpXET2转录本的组织定位表明其在整个幼嫩组织中积累，这与编码的蛋白质在生长过程中参与细胞壁修饰过程的作用一致。有人提出FpXET1是高羊茅组织伸长和叶片生长的潜在标记。
