Boatright J H, Knox B E, Jones K M, Stodulkova E, Nguyen H T, Padove S A, Borst D E, Nickerson J M
Department of Ophthalmology, Emory Eye Center, Atlanta, University of Emory, GA 30322, USA.
FEBS Lett. 2001 Aug 24;504(1-2):27-30. doi: 10.1016/s0014-5793(01)02662-x.
The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblastoma and neuroblastoma cells and in vivo experiments with transgenic Xenopus laevis indicate that -1783/+101 and -156/+101 IRBP gene fragments directed expression predominantly to the retina and pineal, with minor neuronal expression elsewhere. In contrast, a -70/+101 fragment was less restrictive in controlling expression, exhibiting activity not only in retina, but also in forebrain, hindbrain, spinal cord, and motor neurons innervating gills.
视网膜间视黄醇结合蛋白（IRBP）的表达仅限于视网膜的光感受器细胞和松果体的松果体细胞。我们试图确定小鼠IRBP 5'侧翼区域中这种受限活性所需的顺式元件。视网膜母细胞瘤和神经母细胞瘤细胞的体外瞬时转染以及转基因非洲爪蟾的体内实验表明，-1783 / +101和-156 / +101 IRBP基因片段主要将表达导向视网膜和松果体，在其他地方有少量神经元表达。相比之下，-70 / +101片段在控制表达方面限制较小，不仅在视网膜中表现出活性，而且在前脑、后脑、脊髓和支配鳃的运动神经元中也有活性。
