Manjarrez E, Rocha T, Rojas-Piloni G, Méndez I, Vélez D, Flores A
Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, 14 sur 6301, Col. San Manuel, Apartado Postal 406., Puebla, Pue. CP 72570, México.
Neuroreport. 2001 Aug 28;12(12):2667-71. doi: 10.1097/00001756-200108280-00016.
The effect produced by the nitric oxide donor SIN-1 on monosynaptic reflexes was examined. Experiments were performed on anesthetized, paralyzed and spinalized cats. Lumbar monosynaptic reflexes were produced by stimulation of Ia afferents. I.v. application of SIN-1 (500 microg/kg) produced a mean marked potentiation of 704% of pre-drug control (100%) in the amplitude of monosynaptic reflexes. In addition, in other experiments a concentration-dependent effect on the amplitude of monosynaptic reflexes was observed after microinjections of SIN-1 into the ventral horn (1 microl; 10(-12) - 10(-3) M), with a mean facilitatory effect of 355%. In both cases, the potentiation was reversible 45 min after i.v. or local application of SIN-1. These results provide the first evidence that monosynaptic reflexes can be potentiated by nitric oxide.
研究了一氧化氮供体SIN-1对单突触反射的影响。实验在麻醉、麻痹和脊髓横断的猫身上进行。通过刺激Ia传入神经产生腰段单突触反射。静脉注射SIN-1（500微克/千克）使单突触反射幅度平均显著增强至药物前对照（100%）的704%。此外，在其他实验中，将SIN-1微量注射到腹角（1微升；10^(-12) - 10^(-3) M）后，观察到对单突触反射幅度的浓度依赖性效应，平均促进效应为355%。在这两种情况下，静脉注射或局部应用SIN-1 45分钟后，增强作用都是可逆的。这些结果首次证明一氧化氮可增强单突触反射。
