Mendz G L, Trend M A
School of Biochemistry and Molecular Genetics, The University of New South Wales, Sydney, Australia.
Redox Rep. 2001;6(3):179-81. doi: 10.1179/135100001101536292.
Metronidazole and glutathione reduction activities were measured in situ in the micro-aerophilic bacteria Campylobacter coli and Helicobacter pylori employing 14N- and 1H-nuclear magnetic resonance spectroscopy. The properties of these enzyme activities were investigated in matched pairs of strains with sensitive and resistant phenotypes to the antimicrobial metronidazole. The results indicated that the ability of each type of strain to reduce metronidazole corresponded to its sensitive or resistance phenotype. Higher levels of glutathione reduction and a significantly lower Ki for metronidazole were observed in sensitive strains compared to resistant strains. These findings suggested a relationship between the cellular machinery regulating intracellular redox status in C. coli and H. pylori, and the effects of metronidazole on these bacteria, which supported the 'scavenging of oxygen' hypothesis.
采用14N和1H核磁共振光谱法，对微需氧菌空肠弯曲菌和幽门螺杆菌中的甲硝唑和谷胱甘肽还原活性进行了原位测定。在对甲硝唑具有敏感和耐药表型的配对菌株中研究了这些酶活性的特性。结果表明，每种菌株还原甲硝唑的能力与其敏感或耐药表型相对应。与耐药菌株相比，敏感菌株中谷胱甘肽还原水平更高，甲硝唑的Ki值显著更低。这些发现表明，空肠弯曲菌和幽门螺杆菌中调节细胞内氧化还原状态的细胞机制与甲硝唑对这些细菌的影响之间存在关联，这支持了“清除氧气”假说。
