Nuclear liver glycoproteins at different stages of chicken embryo development.

In order to examine whether the patterns of nuclear and chromatin glycoproteins change during development the glycoproteins of foetal and adult chicken liver were investigated. Nuclear and chromatin proteins from both sources were separated by SDS-PAGE, transferred onto Immobilon-P transfer membrane or nitrocellulose and tested for concanavalin A (Con A), Galanthus nivalis agglutinin (GNA) and Aleuria aurantia agglutinin (AAA) binding. Results revealed a similarity in the profiles of nuclear and chromatin glycoproteins recognized by Con A from 14-, 16-, 18-day foetal and adult chicken liver. Generally GNA and AAA reacted more weakly with glycoproteins from foetal liver compared with the same glycoproteins from adult liver.