用于扫描电子显微镜研究的小鼠视网膜中视紫红质的血蓝蛋白-抗体标记
Hemocyanin - antibody labeling of phodopsin in mouse retina for a scanning electron microscope study.
作者信息
Jan L Y, Revel J P
出版信息
J Supramol Struct. 1975;3(1):61-6. doi: 10.1002/jss.400030107.
PMID:1152468
Abstract
To reveal the presence of rhodopsin on the surface of the mouse retina, a scanning electron microscope study of the immunolabeling of rhodopsin was attempted. The glutaraldehyde-fixed mouse retina was treated first with rabbit antibodies specific against bovine rhodopsin and then with hemocyanin-labeled goat antibodies specific against rabbit antibody. The distribution of hemocyanin label on mouse retina and the technique used for labeling are discussed.
摘要
为了揭示视紫红质在小鼠视网膜表面的存在情况，尝试对视紫红质免疫标记进行扫描电子显微镜研究。将经戊二醛固定的小鼠视网膜先用针对牛视紫红质的兔抗体处理，然后用针对兔抗体的血蓝蛋白标记的山羊抗体处理。讨论了血蓝蛋白标记在小鼠视网膜上的分布以及用于标记的技术。
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