Bubis J J, Wolman M
J Supramol Struct. 1975;3(1):90-101. doi: 10.1002/jss.400030110.
Bovine cerebral white matter homogenates were oxidized by various oxidizing solutions of equal molarity and subsequently extracted with water. Most of the oxidants tested (K-dichromate, FeCL3, H2O2, O2, and chloroperbenzoic, ascorbic, performic, and periodic acids) rendered the various myelin constituents less extractable than the constituents of unoxidized control homogenates. KMnO4, and to a lesser degree hemoglobin, rendered myelin constituents more extractable with water than those of the control homogenates. The findings indicate that most of the oxidants produced stabilization of the lamellar pattern, probably through cross-linking and polymerization. KMnO4 and hemoglobin caused labilization and breakdown of the membranous structure. Proof that stabilization of membranes is caused by some oxidants and that fragmentation occurs by the action of KMnO4 and hemoglobin was obtained by the light scattering technique and by electron microscopy of the oxidized homogenates. Evidence obtained indicated that formation of hydrophobic end groups during oxidation favors polymerization, while prevalence of hydrophilic groups is associated with fragmentation.
用等摩尔浓度的各种氧化溶液对牛脑白质匀浆进行氧化，随后用水萃取。所测试的大多数氧化剂（重铬酸钾、氯化铁、过氧化氢、氧气以及氯过苯甲酸、抗坏血酸、过甲酸和高碘酸）使各种髓磷脂成分的可萃取性低于未氧化对照匀浆中的成分。高锰酸钾，以及程度稍轻的血红蛋白，使髓磷脂成分比对照匀浆中的成分更易被水萃取。研究结果表明，大多数氧化剂可能通过交联和聚合作用使层状结构稳定。高锰酸钾和血红蛋白导致膜结构不稳定并分解。通过光散射技术和对氧化匀浆的电子显微镜观察，证实了某些氧化剂会导致膜稳定，而高锰酸钾和血红蛋白的作用会导致膜碎片化。所获得的证据表明，氧化过程中疏水端基的形成有利于聚合，而亲水基团占优势则与碎片化有关。
