Russell W M, Klaenhammer T R
Department of Microbiology, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, North Carolina 27695, USA.
Appl Environ Microbiol. 2001 Sep;67(9):4361-4. doi: 10.1128/AEM.67.9.4361-4364.2001.
An efficient method is described for the generation of site-specific chromosomal integrations in Lactobacillus acidophilus and Lactobacillus gasseri. The strategy is an adaptation of the lactococcal pORI system (K. Leenhouts, G. Venema, and J. Kok, Methods Cell Sci. 20:35-50, 1998) and relies on the simultaneous use of two plasmids. The functionality of the integration strategy was demonstated by the insertional inactivation of the Lactobacillus acidophilus NCFM lacL gene encoding beta-galactosidase and of the Lactobacillus gasseri ADH gusA gene encoding beta-glucuronidase.
描述了一种在嗜酸乳杆菌和加氏乳杆菌中产生位点特异性染色体整合的有效方法。该策略是对乳球菌pORI系统(K. Leenhouts、G. Venema和J. Kok,《细胞科学方法》20:35 - 50,1998)的改进,依赖于同时使用两种质粒。通过嗜酸乳杆菌NCFM编码β-半乳糖苷酶的lacL基因和加氏乳杆菌ADH编码β-葡萄糖醛酸酶的gusA基因的插入失活,证明了整合策略的功能。