Kleeman E G, Klaenhammer T R
J Dairy Sci. 1982 Nov;65(11):2063-9. doi: 10.3168/jds.S0022-0302(82)82462-4.
Thirty-two lactobacilli were tested for ability to adhere to a human fetal intestinal epithelial cell line. By an in vitro system, two adherence mechanisms were found. One mechanism, requiring calcium in the adherence reaction, was nonspecific and allowed all lactobacilli tested to adhere. The other system, not requiring calcium, was found in four strains, all human Lactobacillus acidophilus isolates. Colonial morphology, serial broth passage, and exposure of cell crops to freezing or lyophilization did not affect adherence of Lactobacillus acidophilus. In vitro adherence, combined with subsequent in vivo studies, may provide a basis for screening candidate organisms for use in microbiotic supplements.
对32株乳酸杆菌进行了黏附人胎儿肠道上皮细胞系能力的测试。通过体外系统，发现了两种黏附机制。一种机制在黏附反应中需要钙，是非特异性的，能使所有测试的乳酸杆菌黏附。另一种系统不需要钙，在4株菌株中发现，均为人嗜酸乳杆菌分离株。嗜酸乳杆菌的菌落形态、连续肉汤传代以及细胞培养物的冷冻或冻干处理均不影响其黏附。体外黏附，结合后续的体内研究，可为筛选用于微生物补充剂的候选生物提供依据。
