Fahrer A M, Konigshofer Y, Kerr E M, Ghandour G, Mack D H, Davis M M, Chien Y H
Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10261-6. doi: 10.1073/pnas.171320798.
gammadelta T lymphocytes in the intestinal intraepithelial layer (gammadelta IELs) are thought to contribute to immune competence, but their actual function remains poorly understood. Here we used DNA microarrays to study the gene expression profile of gammadelta IELs in a Yersinia infection system to better define their roles. To validate this approach, mesenteric lymph node CD8(+) alphabeta T cells were similarly analyzed. The transcription profiles show that, whereas lymph node CD8(+) alphabeta T cells must be activated to become cytotoxic effectors, gammadelta IELs are constitutively activated and appear to use different signaling cascades. Our data suggest that gammadelta IELs may respond efficiently to a broad range of pathological situations irrespective of their diverse T cell antigen receptor repertoire. gammadelta IELs may modulate local immune responses and participate in intestinal lipid metabolism, cholesterol homeostasis, and physiology. This study provides a strong basis for further investigations of the roles of these cells as well as mucosal immune defense in general.
肠道上皮内的γδ T淋巴细胞（γδ IELs）被认为有助于免疫功能，但它们的实际功能仍知之甚少。在这里，我们使用DNA微阵列研究γδ IELs在耶尔森菌感染系统中的基因表达谱，以更好地确定它们的作用。为了验证这种方法，我们对肠系膜淋巴结CD8(+) αβ T细胞进行了类似的分析。转录谱显示，虽然淋巴结CD8(+) αβ T细胞必须被激活才能成为细胞毒性效应器，但γδ IELs是组成性激活的，并且似乎使用不同的信号级联。我们的数据表明，γδ IELs可能对广泛的病理情况做出有效反应，而不管它们多样的T细胞抗原受体库如何。γδ IELs可能调节局部免疫反应，并参与肠道脂质代谢、胆固醇稳态和生理功能。这项研究为进一步研究这些细胞的作用以及一般的黏膜免疫防御提供了有力的基础。
