天然型和硒代蛋氨酸CcmG（CycY，DsbE）的结晶及初步衍射研究
Crystallization and preliminary diffraction studies of native and selenomethionine CcmG (CycY, DsbE).
作者信息
Edeling M A, Guddat L W, Fabianek R A, Halliday J A, Jones A, Thöny-Meyer L, Martin J L
机构信息
Centre for Drug Design and Development and Special Research Centre for Functional and Applied Genomics, Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia.
出版信息
Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1293-5. doi: 10.1107/s0907444901009982. Epub 2001 Aug 23.
Disulfide-bond (Dsb) proteins are a family of redox proteins containing a Cys-X-X-Cys motif. They are essential for disulfide-bond exchange in the bacterial periplasm and are necessary for the correct folding and function of many secreted proteins. CcmG (DsbE) is a reducing Dsb protein required for cytochrome c maturation. Crystals of Bradyrhizobium japonicum CcmG have been obtained that diffract X-rays to 1.14 A resolution. The crystals are orthorhombic, space group P2(1)2(1)2(1), with unit-cell parameters a = 35.1, b = 48.2, c = 90.2 A. Selenomethionine CcmG was expressed without using a methionine auxotroph or methionine-pathway inhibition and was purified without reducing agents.
二硫键（Dsb）蛋白是一类含有Cys-X-X-Cys基序的氧化还原蛋白。它们对于细菌周质中的二硫键交换至关重要，并且是许多分泌蛋白正确折叠和发挥功能所必需的。CcmG（DsbE）是细胞色素c成熟所需的一种还原性Dsb蛋白。已获得日本慢生根瘤菌CcmG的晶体，其X射线衍射分辨率达到1.14 Å。晶体为正交晶系，空间群为P2(1)2(1)2(1)，晶胞参数a = 35.1、b = 48.2、c = 90.2 Å。硒代甲硫氨酸CcmG在不使用甲硫氨酸营养缺陷型或甲硫氨酸途径抑制的情况下表达，并在不使用还原剂的情况下进行纯化。
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