正常、胰腺切除及四氧嘧啶糖尿病犬血浆胰高血糖素免疫反应性的异质性

Heterogeneity of plasma glucagon immunoreactivity in normal, depancreatized, and alloxan-diabetic dogs.

作者信息

Valverde I, Dobbs R, Unger R H

出版信息

Metabolism. 1975 Sep;24(9):1021-8. doi: 10.1016/0026-0495(75)90095-5.

Abstract

Filtration of basal plasma from normal, alloxan-diabetic, and depancreatized dogs on Bio Gel P-10 yielded four glucagon-immunoreactive fractions. One of them appeared in the true glycagon area with the glucagon-125I (3500 mol vt). Of the other three, one appeared in the void volume (greater than 20000 mol wt), another just before the insulin-125I (congruent to 9000 mol wt), and the last one close to the salt peak (less than 2000 mol wt). The increase of total plasma glucagon immunoreactivity observed in depancreatized and alloxan diabetic dogs was mainly due to an increase in the 3500 and 9000 molecular-weight fractions. Arginine infusion in depancreatized dogs caused an increase in the 3500 molecular-weight fraction. Somatostatin or insulin infusion in depancreatized and alloxan-diabetic dogs resulted in disappearance of the 3500 molecular-weight fraction.

摘要

对正常犬、四氧嘧啶糖尿病犬和胰腺切除犬的基础血浆在生物凝胶P - 10上进行过滤，得到了四个具有胰高血糖素免疫反应性的组分。其中一个出现在与125I标记的胰高血糖素（3500摩尔分子量）相同的真性胰高血糖素区域。另外三个组分中，一个出现在空体积（大于20000摩尔分子量），另一个恰好在125I标记的胰岛素之前出现（约9000摩尔分子量），最后一个靠近盐峰（小于2000摩尔分子量）。在胰腺切除犬和四氧嘧啶糖尿病犬中观察到的血浆总胰高血糖素免疫反应性增加主要是由于分子量为3500和9000的组分增加。对胰腺切除犬输注精氨酸导致分子量为3500的组分增加。对胰腺切除犬和四氧嘧啶糖尿病犬输注生长抑素或胰岛素导致分子量为3500的组分消失。