Shiras A, Sengupta A, Shepal V
National Centre for Cell Science (NCCS), NCCS Complex, Ganeshkhind, Pune, 411007, India.
Mol Cell Biol Res Commun. 2001 Sep;4(5):313-9. doi: 10.1006/mcbr.2001.0298.
Dlxin-1, a unique member of the necdin/melanoma associated antigen gene (MAGE) family, is a novel protein that binds Dlxin-5 and regulates its transcriptional function. We have cloned the homology region between Dlxin-1 and necdin from mouse melanoma cells. Here we report the expression cloning, characterization, and detailed tissue-specific expression studies of Dlxin-1. A unique expression pattern of Dlxin-1 emerged from the work wherein strong expression of a 3.2-Kb transcript was observed in mouse brain and embryos. Amongst the representative established cell lines of different tumor categories studied the presence of transcript was detected only in sarcomas and neuroectodermal tumors. Characteristically, lymphomas, leukaemias, adenocarcinomas, and carcinomas did not express Dlxin-1. Also, we observed a growth suppression on ectopic expression of this cDNA possibly due to the close homology shared with necdin, a neuron-specific growth suppressor. The extensive homology of our Dlxin-1 clone to necdin makes it an attractive system to understand the importance of the necdin/MAGE family of molecules in cell cycle regulation.
Dlxin-1是necdin/黑色素瘤相关抗原基因（MAGE）家族的独特成员，是一种能与Dlxin-5结合并调节其转录功能的新型蛋白质。我们从小鼠黑色素瘤细胞中克隆了Dlxin-1与necdin之间的同源区域。在此，我们报告Dlxin-1的表达克隆、特性鉴定以及详细的组织特异性表达研究。Dlxin-1呈现出独特的表达模式，在该研究中，在小鼠脑和胚胎中观察到3.2-Kb转录本的强表达。在所研究的不同肿瘤类型的代表性已建立细胞系中，仅在肉瘤和神经外胚层肿瘤中检测到转录本的存在。典型的是，淋巴瘤、白血病、腺癌和癌均不表达Dlxin-1。此外，我们观察到该cDNA异位表达时会抑制生长，这可能是由于其与神经元特异性生长抑制因子necdin具有密切同源性所致。我们的Dlxin-1克隆与necdin的广泛同源性使其成为了解necdin/MAGE分子家族在细胞周期调控中重要性的有吸引力的系统。
