Hattori H, Okano M, Yoshino T, Akagi T, Nakayama E, Saito C, Satoskar A R, Ogawa T, Azuma M, Nishizaki K
Department of Otolaryngology, Okayama University Medical School, Okayama, Japan.
Clin Exp Allergy. 2001 Aug;31(8):1242-9. doi: 10.1046/j.1365-2222.2001.01021.x.
B7 molecules (CD80, CD86) and their counter-receptors, CD28 and CD152 (CTLA-4), play an important role in T cell-mediated immune responses. We previously demonstrated that B7 molecules are selectively up-regulated not only on B cells but also on T cells from the peripheral blood mononuclear cells of patients with perennial rhinitis cultured with allergen. However, the expression of CD80/CD86 molecules and their counter-receptors in nasal mucosa, the actual inflammatory site of allergic rhinitis, has not yet been clarified.
Inferior turbinates from patients with either allergy to house dust or non-allergic rhinitis were excised and immunohistologically stained. In addition, the inferior turbinates were challenged with paper discs containing extracts of house dust and subsequently excised. Samples were double stained with immunofluorescent-labelled antibody to identify cells bearing CD86.
Without the nasal provocation, only the expression of CD86 was increased in nasal mucosa of patients with allergic rhinitis compared with those with non-allergic rhinitis. However, following the nasal provocation with house dust, not only CD86, but also CD80, CD28, and CD152 were significantly expressed in allergic patients. Immunofluorescent double staining revealed CD86 expression in CD19, CD1a, CD14 and CD3 lymphocytes.
These results indicate that the expression of CD80/CD86 molecules and their counter-receptors is induced in allergic patients following nasal provocation with allergen, suggesting a local amplification of allergen-specific immune responses in perennial rhinitis.
B7分子（CD80、CD86）及其对应受体CD28和CD152（CTLA-4）在T细胞介导的免疫反应中起重要作用。我们之前证明，在用过敏原培养的常年性鼻炎患者外周血单个核细胞中，B7分子不仅在B细胞上选择性上调，在T细胞上也上调。然而，变应性鼻炎实际炎症部位鼻黏膜中CD80/CD86分子及其对应受体的表达尚未阐明。
切除对屋尘过敏患者或非变应性鼻炎患者的下鼻甲并进行免疫组织化学染色。此外，用含屋尘提取物的纸盘刺激下鼻甲，随后切除。样本用免疫荧光标记抗体进行双重染色，以识别表达CD86的细胞。
在未进行鼻腔激发时，与非变应性鼻炎患者相比，变应性鼻炎患者鼻黏膜中仅CD86的表达增加。然而，在用屋尘进行鼻腔激发后，变应性鼻炎患者不仅CD86，而且CD80、CD28和CD152均显著表达。免疫荧光双重染色显示CD86在CD19、CD1a、CD14和CD3淋巴细胞中表达。
这些结果表明，变应性鼻炎患者在鼻腔用过敏原激发后，CD80/CD86分子及其对应受体的表达被诱导，提示常年性鼻炎中变应原特异性免疫反应的局部放大。
