脐带血和成人血单核细胞对肝细胞生长因子的反应性差异
Differential responsiveness of cord and adult blood monocytes to hepatocyte growth factor.
作者信息
Jiang Q, Azuma E, Tanaka M, Kobayashi M, Hirayama M, Kumamoto T, Iwamoto S, Yamamoto H, Nakashima K, Sakurai M, Komada Y
机构信息
Department of Paediatrics, Mie University School of Medicine, Japan.
出版信息
Clin Exp Immunol. 2001 Aug;125(2):222-8. doi: 10.1046/j.1365-2249.2001.01591.x.
Monocytes as antigen-presenting cells play an important role in host defence. There are several cytokines affecting monocyte function. We demonstrate that both adult and cord blood monocytes constitutively express hepatocyte growth factor (HGF) receptor, MET. HGF significantly down-regulated MET expression of adult blood monocytes, compared with cord blood monocytes, when cultured either in RPMI-1640 containing 10% FBS or serum-free medium. Surface levels of MET correlated with c-met mRNA levels both in adult and cord blood when cultured. MET expression was down-regulated by treating with actinomycin D or cycloheximide. HGF stimulated DNA synthesis of adult monocytes, but not cord blood. HGF enhanced antigen-presenting capacity of adult blood monocytes but not cord blood monocytes. HGF up-regulated HLA class I expression in adult monocytes but not in cord blood monocytes. The current results suggest that the failure of cord blood monocytes to respond to HGF may be responsible, in large part, for their functional immaturity.
单核细胞作为抗原呈递细胞在宿主防御中发挥着重要作用。有几种细胞因子会影响单核细胞的功能。我们证明，成人和脐带血单核细胞均组成性表达肝细胞生长因子（HGF）受体MET。当在含有10%胎牛血清的RPMI-1640培养基或无血清培养基中培养时，与脐带血单核细胞相比，HGF显著下调成人血液单核细胞的MET表达。培养时，成人和脐带血中MET的表面水平与c-met mRNA水平相关。用放线菌素D或环己酰亚胺处理可下调MET表达。HGF刺激成人单核细胞的DNA合成，但不刺激脐带血单核细胞。HGF增强成人血液单核细胞的抗原呈递能力，但不增强脐带血单核细胞的抗原呈递能力。HGF上调成人单核细胞中HLA I类分子的表达，但不上调脐带血单核细胞中HLA I类分子的表达。目前的结果表明，脐带血单核细胞对HGF无反应可能在很大程度上导致了它们的功能不成熟。
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