Yang B S, Im G S, Park S J
National Livestock Research Institute, 441-350, Suwon, South Korea.
Anim Reprod Sci. 2001 Sep 15;67(3-4):153-8. doi: 10.1016/s0378-4320(01)00125-7.
The main objectives of this investigation were to monitor the birth weight of calves and gestation length following artificial insemination (AI) and transfer of in vivo or in vitro produced Korean native, Hanwoo embryos. Embryos produced in vivo were recovered from uterine flushings of superovulated cows 7 days after AI. Those embryos produced in vitro were co-cultured with cumulus cells for 7-8 days after in vitro fertilization. The birth weights of calves following the transfer of in vitro produced (IVP) embryos were heavier than calves from both of AI- and in vivo-derived embryo transferred calves in both sexes (29.6, 24.1 and 25.2kg, respectively, P<0.05). The IVP calves also had a longer gestation length (293.9, 285.8 and 283.8 days, respectively, P<0.05).
本研究的主要目的是监测人工授精(AI)以及体内或体外生产的韩国本土韩牛胚胎移植后犊牛的出生体重和妊娠期长度。体内生产的胚胎在人工授精7天后从超排母牛的子宫冲洗液中回收。体外生产的胚胎在体外受精后与卵丘细胞共培养7 - 8天。移植体外生产(IVP)胚胎后出生的犊牛,无论雌雄,其出生体重均高于移植AI来源和体内来源胚胎的犊牛(分别为29.6、24.1和25.2千克,P<0.05)。IVP犊牛的妊娠期也更长(分别为293.9、285.8和283.8天,P<0.05)。