Hill M, Bicíková M, Parízek A, Havlíková H, Klak J, Fajt T, Meloun M, Cibula D, Cegan A, Sulcová J, Hampl R, Stárka L
Institute of Endocrinology, Národní trída 8, CZ 116 94, Prague, Czech Republic.
J Steroid Biochem Mol Biol. 2001 Jul;78(1):51-7. doi: 10.1016/s0960-0760(01)00073-5.
Time profiles of the pregnanolone isomers epipregnanolone (3 beta-hydroxy-5 beta-pregnan-20-one), allopregnanolone (3 alpha-hydroxy-5 alpha-pregnan-20-one), pregnanolone (3 alpha-hydroxy-5 beta-pregnan-20-one), and isopregnanolone (3 beta-hydroxy-5 alpha-pregnan-20-one) were measured around parturition and in the postpartum period in the serum of 13 and three women with subarachnoidal and epidural analgesia, respectively. In addition, the levels of polar conjugates of all pregnanolone isomers were followed during parturition. GC/MS analysis was used for the measurement of steroid levels. Changes in concentrations of free steroids exhibited a similar pattern, with a fall primarily within the first hour after delivery. The decrease in conjugated steroids was shifted to the interval within the first hour and first day after delivery, and the changes were more pronounced. The time profile of the conjugated/free steroid ratio exhibited a significant decrease within the first hour and the first day after delivery in all of the isomers investigated. A decrease was also observed in the ratio of 3 alpha/3 beta-isomers and 5 alpha/5 beta-isomers around parturition. The possible physiological consequences of the findings are indicated.
在13名接受蛛网膜下腔镇痛和3名接受硬膜外镇痛的产妇分娩前后及产后期间，测定了孕烷醇酮异构体表孕烷醇酮（3β-羟基-5β-孕烷-20-酮）、别孕烷醇酮（3α-羟基-5α-孕烷-20-酮）、孕烷醇酮（3α-羟基-5β-孕烷-20-酮）和异孕烷醇酮（3β-羟基-5α-孕烷-20-酮）的血清时间曲线。此外，在分娩期间对所有孕烷醇酮异构体的极性共轭物水平进行了跟踪。采用气相色谱/质谱分析法测定类固醇水平。游离类固醇浓度的变化呈现出相似的模式，主要在分娩后第一小时内下降。共轭类固醇的下降转移至分娩后第一小时和第一天的时间段内，且变化更为明显。在所有研究的异构体中，共轭/游离类固醇比值的时间曲线在分娩后第一小时和第一天内呈现出显著下降。在分娩前后还观察到3α/3β异构体和5α/5β异构体的比值下降。文中指出了这些发现可能的生理后果。
