[采用MTT法体外评价抗腺病毒药物]

[Evaluation of antiviral agents for adenovirus using the MTT method in vitro].

作者信息

Kaneko H, Fujiwara T, Mori S, Shigeta S

机构信息

Department of Ophthalmology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 2000 Nov;104(11):786-91.

PMID:11530368

Abstract

PURPOSE

Currently, there is no effective treatment for adenoviral conjunctivitis. We evaluated the antiviral inhibitory effect of four antiviral agents against adenovirus (ADV) in vitro.

METHODS

Viruses used for the experiment were ADV type 4 (ADV 4), type 8 (ADV 8) and type 37 (ADV 37). We examined four antiviral agents, i.e., cidofovir (HPMPC), zalcitabine (ddC), foscarnet (PFA), and acyclovir (ACV). 50% effective concentration (EC50), 50% cytotoxic concentration (CC50) and selectivity index (SI) of compounds were determined for ADV infection in HEp-2 cells using the 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.

RESULTS

HPMPC and ddC showed an inhibitory effect against all three ADVs. In particular, ddC showed more potent and selective inhibition against ADV than HPMPC. PFA and ACV were ineffective against ADV.

CONCLUSIONS

HPMPC and ddC were inhibitory against ADV 4, ADV 8 and ADV 37 replication in vitro. The MTT method is rapid and simple for the screening of antiviral agents. We think this method is also very useful for the screening of anti-ADV agents.

摘要

目的

目前，腺病毒性结膜炎尚无有效的治疗方法。我们在体外评估了四种抗病毒药物对腺病毒（ADV）的抗病毒抑制作用。

方法

用于实验的病毒为4型腺病毒（ADV 4）、8型腺病毒（ADV 8）和37型腺病毒（ADV 37）。我们检测了四种抗病毒药物，即西多福韦（HPMPC）、扎西他滨（ddC）、膦甲酸钠（PFA）和阿昔洛韦（ACV）。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定化合物对HEp-2细胞中ADV感染的50%有效浓度（EC50）、50%细胞毒性浓度（CC50）和选择性指数（SI）。