Krasil'nikova E N, Tsaplina I A, Zakharchuk L M, Bogdanova T I
M. V. Lomonosov, Moscow State University, Moscow, 117312 Russia.
Prikl Biokhim Mikrobiol. 2001 Jul-Aug;37(4):418-23.
Aerobic thermoacidophilic chemolithotrophic bacteria Sulfobacillus thermosulfidooxidans 1269T and Sulfobacillus thermosulfidooxidans subsp. asporogenes 41 were shown to be resistant to stress factors, including high concentrations of Zn2+ (0.8 M) and H+ (pH 1.2) that exceeded the optimum values. The growth and biomass gain rates decreased, but bacteria retained their functions. The activity of nearly all enzymes involved in carbon metabolism decreased. Glucose was primarily metabolized via the Entner--Doudoroff pathway. The activity tricarboxylic acid cycle enzymes decreased compared to that in cells grown under normal conditions. After saturation of the growth medium with 5 vol % CO2, sulfobacteria utilized glucose by the Embden-Meyerhof and pentose phosphate pathways under mixotrophic conditions.
需氧嗜热嗜酸化能自养细菌嗜热硫化氧化硫杆菌1269T和嗜热硫化氧化硫杆菌亚种产芽孢硫杆菌41被证明对包括高浓度Zn2+（0.8 M）和H+（pH 1.2）在内的应激因素具有抗性，这些浓度超过了最佳值。生长和生物量增加速率降低，但细菌仍保留其功能。几乎所有参与碳代谢的酶的活性均下降。葡萄糖主要通过恩特纳-杜德洛夫途径代谢。与在正常条件下生长的细胞相比，三羧酸循环酶的活性降低。在用5体积% CO2使生长培养基饱和后，硫细菌在混合营养条件下通过糖酵解途径和磷酸戊糖途径利用葡萄糖。
