Edwards M R, Collins A M, Ward R L
School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.
Curr Pharm Biotechnol. 2001 Sep;2(3):225-40. doi: 10.2174/1389201013378653.
The ability to display IgE antibody fragments, allergens and peptides upon filamentous phage has increasingly been used in allergy research. This technique offers the opportunity to isolate and produce IgE antibody fragments specific for allergens. These antibody fragments can then be used to address fundamental issues regarding the development of IgE antibodies in allergic patients, at both the molecular and structural level. Random peptide display has greatly facilitated the discovery of epitopes recognized by serum IgE antibodies from allergic patients, and it is a definitive tool for investigating the IgE-epitope interaction. Whole allergens can also be displayed on phage. Selecting IgE binding phage from diverse cDNA libraries of allergens has assisted in the identification of new allergens and provided a source of purified allergens for the diagnosis of allergic diseases. Finally, phage display of antibody fragments and random peptides is currently providing a means by which the IgE antibody can be targeted as a potential treatment for allergy. This review highlights several studies which have utilized phage display methodology in the area of allergy research, and it discusses how the therapeutic potential of this approach may be exploited.
丝状噬菌体展示IgE抗体片段、过敏原和肽的能力在过敏研究中的应用越来越广泛。这项技术为分离和生产针对过敏原的IgE抗体片段提供了机会。这些抗体片段随后可用于解决过敏患者中IgE抗体在分子和结构水平上的产生所涉及的基本问题。随机肽展示极大地促进了对过敏患者血清IgE抗体识别的表位的发现，并且是研究IgE-表位相互作用的决定性工具。完整的过敏原也可以展示在噬菌体上。从不同的过敏原cDNA文库中筛选IgE结合噬菌体有助于鉴定新的过敏原，并为过敏性疾病的诊断提供纯化过敏原的来源。最后，抗体片段和随机肽的噬菌体展示目前提供了一种将IgE抗体作为过敏潜在治疗靶点的方法。本综述重点介绍了在过敏研究领域利用噬菌体展示方法的几项研究，并讨论了如何利用这种方法的治疗潜力。
