Matoga M, Pehourcq F, Titier K, Dumora F, Jarry C
Pharmacochimie, EA 2962, Université Bordeaux 2 Victor Segalen, France.
J Chromatogr B Biomed Sci Appl. 2001 Sep 5;760(2):213-8. doi: 10.1016/s0378-4347(01)00270-5.
Venlafaxine, a second-generation antidepressant, acts by inhibition of the reuptake of presynaptic noradrenaline and serotonin. The main metabolite, O-desmethylvenlafaxine was found biologically active. For toxicological purpose, a rapid specific and accurate RP-HPLC assay was developed for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma. A linear response was observed over the concentration range 0.2-4 microg/ml. A good accuracy (<8%) was achieved for all quality controls, with intra-day and inter-day variation coefficient less than 10%. Finally, no interference was observed with other psychotic drugs encountered in acute poisoning. This rapid method (run time <10 min) was used to manage four voluntary intoxications involving venlafaxine.
文拉法辛是一种第二代抗抑郁药，通过抑制突触前去甲肾上腺素和5-羟色胺的再摄取发挥作用。其主要代谢产物O-去甲基文拉法辛具有生物活性。出于毒理学目的，开发了一种快速、特异且准确的反相高效液相色谱法（RP-HPLC），用于同时测定人血浆中的文拉法辛和O-去甲基文拉法辛。在0.2 - 4微克/毫升的浓度范围内观察到线性响应。所有质量控制的准确度良好（<8%），日内和日间变异系数均小于10%。最后，未观察到与急性中毒中遇到的其他精神药物的干扰。这种快速方法（运行时间<10分钟）用于处理四起涉及文拉法辛的自愿中毒事件。
