Kays M B, White R L, Friedrich L V
Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, Purdue University, Indianapolis, IN 46202-2879, USA.
J Antimicrob Chemother. 2001 Sep;48(3):417-20. doi: 10.1093/jac/48.3.417.
We evaluated the effect of serum from normal and uraemic volunteers, neutropenic patients and burn patients on the serum bactericidal test. Serum samples were spiked with ceftazidime to mimic in vivo peak (75 mg/L) and trough (5 mg/L) concentrations. Serum inhibitory and bactericidal titres (SIT and SBT) were performed in triplicate using Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. For E. coli, the trough SIT and SBT were significantly higher in serum from burn patients compared with normal volunteers (P < or = 0.024). The trough SIT was significantly higher in serum from burn patients compared with neutropenic patients (P = 0.022) and in uraemic patients compared with normal volunteers (P = 0.04). No significant differences between subject populations were found for P. aeruginosa.
我们评估了来自正常志愿者、尿毒症患者、中性粒细胞减少症患者和烧伤患者的血清对血清杀菌试验的影响。向血清样本中加入头孢他啶，以模拟体内峰值（75毫克/升）和谷值（5毫克/升）浓度。使用大肠埃希菌ATCC 25922和铜绿假单胞菌ATCC 27853对血清抑菌滴度和杀菌滴度（SIT和SBT）进行了三次重复检测。对于大肠埃希菌，烧伤患者血清中的谷值SIT和SBT显著高于正常志愿者（P≤0.024）。烧伤患者血清中的谷值SIT显著高于中性粒细胞减少症患者（P = 0.022），尿毒症患者血清中的谷值SIT显著高于正常志愿者（P = 0.04）。对于铜绿假单胞菌，未发现不同受试者群体之间存在显著差异。
