酵母Ty5逆转录转座子靶向沉默染色质是由整合酶与Sir4p之间的相互作用介导的。
Targeting of the yeast Ty5 retrotransposon to silent chromatin is mediated by interactions between integrase and Sir4p.
作者信息
Xie W, Gai X, Zhu Y, Zappulla D C, Sternglanz R, Voytas D F
机构信息
Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011-3260, USA.
出版信息
Mol Cell Biol. 2001 Oct;21(19):6606-14. doi: 10.1128/MCB.21.19.6606-6614.2001.
Abstract
The Ty5 retrotransposons of Saccharomyces cerevisiae integrate preferentially into regions of silent chromatin at the telomeres and silent mating loci (HMR and HML). We define a Ty5-encoded targeting domain that spans 6 amino acid residues near the C terminus of integrase (LXSSXP). The targeting domain establishes silent chromatin when it is tethered to a weakened HMR-E silencer, and it disrupts telomeric silencing when it is overexpressed. As determined by both yeast two-hybrid and in vitro binding assays, the targeting domain interacts with the C terminus of Sir4p, a structural component of silent chromatin. This interaction is abrogated by mutations in the targeting domain that disrupt integration into silent chromatin, suggesting that recognition of Sir4p by the targeting domain is the primary determinant in Ty5 target specificity.
摘要
酿酒酵母的Ty5逆转座子优先整合到端粒和沉默交配位点（HMR和HML）的沉默染色质区域。我们定义了一个Ty5编码的靶向结构域，它位于整合酶C末端附近的6个氨基酸残基处（LXSSXP）。当该靶向结构域与弱化的HMR - E沉默子相连时可建立沉默染色质，而当它过度表达时则会破坏端粒沉默。通过酵母双杂交和体外结合试验确定，该靶向结构域与Sir4p的C末端相互作用，Sir4p是沉默染色质的一种结构成分。靶向结构域中破坏整合到沉默染色质中的突变消除了这种相互作用，这表明靶向结构域对Sir4p的识别是Ty5靶标特异性的主要决定因素。
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本文引用的文献
1
Expression and processing of proteins encoded by the Saccharomyces retrotransposon Ty5.酿酒酵母逆转录转座子Ty5编码蛋白的表达与加工
J Virol. 2001 Feb;75(4):1790-7. doi: 10.1128/JVI.75.4.1790-1797.2001.
2
The Brf and TATA-binding protein subunits of the RNA polymerase III transcription factor IIIB mediate position-specific integration of the gypsy-like element, Ty3.RNA聚合酶III转录因子IIIB的Brf和TATA结合蛋白亚基介导了类吉普赛元件Ty3的位置特异性整合。
J Biol Chem. 2000 Sep 22;275(38):29800-7. doi: 10.1074/jbc.M003149200.
3
A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.Sir2蛋白家族中一种系统发育保守的NAD⁺依赖性蛋白脱乙酰酶活性。
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6658-63. doi: 10.1073/pnas.97.12.6658.
4
Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase-RNase H domain exhibits polymerase and RNase H activities.重组Ty1逆转录酶活性形式在大肠杆菌中的表达：一种融合蛋白，其包含与逆转录酶-RNase H结构域相连的Ty1整合酶C末端区域，具有聚合酶和RNase H活性。
Biochem J. 2000 Jun 1;348 Pt 2(Pt 2):337-42.
5
The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases.沉默蛋白SIR2及其同源物是依赖烟酰胺腺嘌呤二核苷酸的蛋白质脱乙酰酶。
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5807-11. doi: 10.1073/pnas.110148297.
6
Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.转录沉默与长寿蛋白Sir2是一种依赖烟酰胺腺嘌呤二核苷酸的组蛋白脱乙酰酶。
Nature. 2000 Feb 17;403(6771):795-800. doi: 10.1038/35001622.
7
Tagging chromatin with retrotransposons: target specificity of the Saccharomyces Ty5 retrotransposon changes with the chromosomal localization of Sir3p and Sir4p.用逆转录转座子标记染色质：酿酒酵母Ty5逆转录转座子的靶标特异性随Sir3p和Sir4p的染色体定位而变化。
Genes Dev. 1999 Oct 15;13(20):2738-49. doi: 10.1101/gad.13.20.2738.
8
Modular evolution of the integrase domain in the Ty3/Gypsy class of LTR retrotransposons.LTR逆转录转座子Ty3/Gypsy类中整合酶结构域的模块化进化。
J Virol. 1999 Jun;73(6):5186-90. doi: 10.1128/JVI.73.6.5186-5190.1999.
9
Polymorphisms and genomic organization of repetitive DNA from centromeric regions of Arabidopsis chromosomes.拟南芥染色体着丝粒区域重复DNA的多态性与基因组组织
Plant Cell. 1999 Jan;11(1):31-42. doi: 10.1105/tpc.11.1.31.
10
Functional characterization of the N terminus of Sir3p.Sir3p蛋白N端的功能特性
Mol Cell Biol. 1998 Oct;18(10):6110-20. doi: 10.1128/MCB.18.10.6110.
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